Efikasi Filtrat Kasar Saponin Biji Keben (Barringtonia asiatica) Terhadap Bakteri Vibrio harveyi
DOI:
https://doi.org/10.34145/agriekstensia.v25i1.3883Keywords:
Barringtonia asiatica, Saponin, Vibrio harveyi, Antibakteri, BiokontrolAbstract
Pengendalian Vibrio harveyi, patogen penyebab luminous vibriosis, masih bergantung pada antibiotik konvensional, yang memicu resistensi antimikroba (AMR). Saponin dari biji Barringtonia asiatica (keben) telah dilaporkan memiliki aktivitas antibakteri. Penelitian ini bertujuan mengevaluasi efektivitas filtrat kasar saponin B. asiatica pada berbagai dosis terhadap V. harveyi. Pengujian dilakukan menggunakan Rancangan Acak Lengkap (RAL) dengan enam perlakuan (A=0,7 ppm, B=0,9 ppm, C=1,1 ppm, D=1,3 ppm, E=1,5 ppm, dan Kontrol=0 ppm), masing-masing tiga ulangan. Kultur V. harveyi (1x106 sel/mL) diinkubasi selama 24 jam bersama filtrat. Efektivitas diukur melalui Total Bacterial Count (TBC) pada media TCBSA dan dihitung sebagai Persentase Penghambatan (%) serta Log Reduction. Filtrat kasar saponin secara signifikan memengaruhi pertumbuhan V. harveyi (p < 0,05). Efektivitas tertinggi dicapai pada dosis E (1,5 ppm), menghasilkan 90,00% penghambatan dan 0,98 Log Reduction. Filtrat kasar saponin biji B. asiatica merupakan agen antibakteri yang efektif terhadap V. harveyi pada dosis tinggi. Filtrat ini memiliki potensi besar sebagai alternatif biokontrol berkelanjutan dalam akuakultur.
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References
APHA (American Public Health Association). (2004). Standard Methods for the Examination of Water and Wastewater (21st ed.). American Public Health Association. http://www.standardmethods.org.
Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in Vitro Evaluating Antimicrobial Activity: A Review. Journal of Pharmaceutical Analysis, 6(2), 71–79. https://doi.org/10.1016/j.jpha.2015.11.002.
Calabrese, E. J., & Baldwin, L. A. (2003). Toxicology and the Dose-Response: A Historical Perspective. Toxicology and Applied Pharmacology, 190(2), 131–138. https://doi.org/10.1016/S0041-008X(03)00241-1.
Cankaya, I. I. T., & Somuncuoglu, E. I. (2021). Potential and Prophylactic Use of Plants Containing Saponin-Type Compounds as Antibiofilm Agents Against Respiratory Tract Infections. Evidence-Based Complementary and Alternative Medicine, 2021, 6814215. https://doi.org/10.1155/2021/6814215.
Cappuccino, J. G., & Welsh, C. T. (2017). Microbiology: A Laboratory Manual (11th ed.). Pearson.
CDC (Centers for Disease Control and Prevention). (2020). Antibiotic Resistance Laboratory and Testing Methods. CDC. https://www.cdc.gov/drugresistance/report/index.html.
Choo, Q. Y., Tan, J. S., & Ooi, M. C. (2021). The Physicochemical Properties of Saponin Extracts from Tropical Plants. Journal of Molecular Liquids, 336, 116348. https://doi.org/10.1016/j.molliq.2021.116348.
Dawood, M. A. O. (2021). Antibiotic Resistance in Aquaculture: Challenges, Trends Analysis, and Alternative Approaches. Antibiotics, 10(6), 598. https://doi.org/10.3390/antibiotics10060598.
FAO (Food and Agriculture Organization of the United Nations). (2022). The State of World Fisheries and Aquaculture. FAO. http://www.fao.org/3/cc0461en/cc0461en.pdf.
FDA (Food and Drug Administration). (2019). Guidance for Industry: Establishing Time Limits for Critical Operations in the Manufacture of Unlicensed Drugs. FDA. https://www.fda.gov/media/132448/download.
Gomez-Gil, B., Roque, A., & Turnbull, J. F. (2020). The Occurrence and Pathogenicity of Vibrio harveyi in Aquaculture. Aquaculture, 219, 45–66. https://doi.org/10.1016/S0044-8486(02)00392-1.
Harborne, J. B. (1999). Classes of Natural Products and Their Distribution. Encyclopedia of Life Sciences, 3, 456–465. https://doi.org/10.1038/npg.els.0001880.
Hostettmann, K., & Marston, A. (1995). Saponins: Chemistry and Pharmacology of Natural Products. Cambridge University Press.
Khare, C. P. (2007). Indian Medicinal Plants: An Illustrated Dictionary. Springer.
Kiran, B. S., Arasu, M. V., & Durairaj, S. (2022). Inhibition of Quorum Sensing and Virulence Factors of Vibrio Species by Plant Extracts: A Mini-Review. Microbial Pathogenesis, 165, 105494. https://doi.org/10.1016/j.micpath.2022.105494.
Liao, P. Y., Wang, P. C., & Yang, H. J. (2018). Triterpenoid Saponins from the Seeds of Barringtonia asiatica. Molecules, 23(11), 2768. https://doi.org/10.3390/molecules23112768.
Liu, M., Zhang, B., & Liu, X. (2021). The Antibacterial Mechanism of Triterpenoid Saponins: A Systematic Review. Phytomedicine, 92, 153787. https://doi.org/10.1016/j.phymed.2021.153787.
Mara, J. M., & Anselmo, C. (2024). Antimicrobial and Cytotoxic Potential of Barringtonia asiatica Seed Extracts: A Multi-Assay Approach. Journal of Interdisciplinary Perspectives, 2(12), 605–618. https://doi.org/10.58421/jips.v2i12.605.
Martínez-Gómez, J. M., Pérez-Sánchez, M. T., & García-Sánchez, M. J. (2023). The Challenge of Laboratory Testing of Antimicrobials Against Marine Bacteria. Marine Biotechnology, 25(3), 401–410. https://doi.org/10.1007/s10126-023-10250-9.
Pikhtirova, A., Pecka Kiełb, E., & Zigo, F. (2023). Antimicrobial Activity of Saponin-Containing Plants: Review. Journal of Dairy, Veterinary & Animal Research, 12(2), 121–127. https://doi.org/10.15406/jdvar.2023.12.00407.
Roca, I., Akova, M., & Baquero, F. (2023). The Global Threat of Antimicrobial Resistance: Science for Intervention. Science, 343, 1450–1459. https://doi.org/10.1126/science.1234478.
Sarkar, S., Mondal, C., & Sen, G. (2022). Low-Dose Effects of Antibiotics: A Review of Hormesis and its Implication in Microbial Evolution. Environmental Microbiology Reports, 14(5), 673–680. https://doi.org/10.1111/1758-2229.13093.
Schulz, H. (2011). Hormesis: A New Paradigm for Risk Assessment, Drug Discovery and Development. Springer.
Sofian, Z., Wibisono, I. T., & Mustofa, K. (2022). The Potential of Barringtonia asiatica Biopesticide from Papua to Eradicate Pests in Aquaculture. Jurnal Airaha, 11(1), 157–166. https://doi.org/10.53676/ja.v11i1.353.
Thomsen, I., & Nørlund, L. (2022). Aquaculture Health Management: A Practical Guide. Wiley-Blackwell.
Wang, T., Shi, X., Sun, W., Huang, Z., Li, Y., Yang, F., & Liu, C. (2012). Quantification of Total Saponins in Gynostemma pentaphyllum by High-Performance Liquid Chromatography. Journal of Separation Science, 35(3), 478–483. https://doi.org/10.1002/jssc.201100780.
Zhang, X., He, X., & Austin, B. (2020). Vibrio harveyi: A Serious Pathogen of Fish and Invertebrates in Mariculture. Marine Life Science & Technology, 2(3), 231–245. https://doi.org/10.1007/s42995-020-00049-5.
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