Peningkatan Kualitas Kit Elisa Rabies Pusvetma
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Abstract
Enzym Linked Immunosorbent Assay (ELISA) merupakan salah satu alat uji untuk mendeteksi antibody. Teknik pengujian atau pemeriksaan berdasarkan atas ikatan antigen antibodi yang mana salah satu antigen atau antibodi dilabel dengan Enzym tertentu dan reaktan lainnya berikatan. Metode yang dilakukan dalam penelitian ini yaitu persiapan mikroplate (coating, blocking, washing) dan pengujian kit ELISA. Hasil uji serum kontrol positif, serum kontrol negatif, serum standart dan serum sampel yang menggunakan substrat label TMB dan Konjugat Protein A-HRP menunjukkan nilai OD yang lebih stabil dibanding dengan yang menggunakan substrat PNPP dan Konjugat Protein A-AP (Alkaline Phosphatase) dengan panjang gelombang 405 nm.
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How to Cite
Mashelli, E., Sjolichah, N., & Hartanti, F. (2022). Peningkatan Kualitas Kit Elisa Rabies Pusvetma. AGRIEKSTENSIA : Jurnal Penelitian Terapan Bidang Pertanian, 21(1), 51-55. https://doi.org/10.34145/agriekstensia.v21i1.2188
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References
Biorad. 2009. User's manual Platelia Rabies II Kit. Tersedia pada: http: //web. oie. Int /VCDA/ eng/ Registre/ User's %20 manual_Platelia Rabies II. pdf.
D. Catty. 1989. A Practical Approach. Vol II
Hooper, D.C., Morimoto, K., Bette, M., Weihe, E., Koprowski, H. dan Dietzschold, B. 1998. Collaboration of Antibody and Inflammation in Clearance of Rabies Virus from The Central Nervous System. J.Virol. 72. 3711-3719.
Johnson, G.A, Zielger, R.J, Hawley, L. 2011. Essential Mikrobiologi dan imunologi. Tangerang selatan: Binarupa Aksara Publisher.
Kang, B., J.S. Oh, C.S. Lee, B.K. Park, Y.N. Park, K.S. Hong, K.G. Lee, B.K. Cho, D.S. Song. 2007. Evaluation of Rapid Immunodiagnostic Test kit for Rabies Virus. Journal of Virology Methods. 145: 30 – 36
OIE. 2011. Terestrial Manual, Rabies. Chapter 2.1.13.
Saputra, P.N., D. Estikoma., dan saN. Shohchah. 2013. Kit ELISA Rabies Pusvetma. Pusat Veteriner Farma. Surabaya.
Sumartini, S., Zuas, O., Juliasmardiany, R., dan Sulilawati, E. 2002. Aplikasi Elisa Kit Untuk Mendeteksi Adanya Daging Babi Dalam Makanan. Pusat Penelitian Kimia; Tangerang
World Health Organization. 1996. Laboratory Techniques in Rabies Fourth Edition, Meslin F-X.Kaplan M.M dan Koprowski H.,Eds. WHO. Geneva. Switzerland.
World Health Organization Expert Commitee on Biological Standards. Thirty-Fifth Report. 1985. World Health Organisation Technical Report Series No. 725. WHO. Geneva. Switzerland.
Xu G., Weber P., Hu Q.,Xue H., Audry L.,Li C., Wu J dan Bourhy H. 2007. A Simple Sanwich Elisa (WELYSSA) For The Detection of Luyssavirus Nucleocapsid in Rabies Suspected Specimens Using Mouse Monoclonal Antibodies Biologicals. 35. 297- 302
D. Catty. 1989. A Practical Approach. Vol II
Hooper, D.C., Morimoto, K., Bette, M., Weihe, E., Koprowski, H. dan Dietzschold, B. 1998. Collaboration of Antibody and Inflammation in Clearance of Rabies Virus from The Central Nervous System. J.Virol. 72. 3711-3719.
Johnson, G.A, Zielger, R.J, Hawley, L. 2011. Essential Mikrobiologi dan imunologi. Tangerang selatan: Binarupa Aksara Publisher.
Kang, B., J.S. Oh, C.S. Lee, B.K. Park, Y.N. Park, K.S. Hong, K.G. Lee, B.K. Cho, D.S. Song. 2007. Evaluation of Rapid Immunodiagnostic Test kit for Rabies Virus. Journal of Virology Methods. 145: 30 – 36
OIE. 2011. Terestrial Manual, Rabies. Chapter 2.1.13.
Saputra, P.N., D. Estikoma., dan saN. Shohchah. 2013. Kit ELISA Rabies Pusvetma. Pusat Veteriner Farma. Surabaya.
Sumartini, S., Zuas, O., Juliasmardiany, R., dan Sulilawati, E. 2002. Aplikasi Elisa Kit Untuk Mendeteksi Adanya Daging Babi Dalam Makanan. Pusat Penelitian Kimia; Tangerang
World Health Organization. 1996. Laboratory Techniques in Rabies Fourth Edition, Meslin F-X.Kaplan M.M dan Koprowski H.,Eds. WHO. Geneva. Switzerland.
World Health Organization Expert Commitee on Biological Standards. Thirty-Fifth Report. 1985. World Health Organisation Technical Report Series No. 725. WHO. Geneva. Switzerland.
Xu G., Weber P., Hu Q.,Xue H., Audry L.,Li C., Wu J dan Bourhy H. 2007. A Simple Sanwich Elisa (WELYSSA) For The Detection of Luyssavirus Nucleocapsid in Rabies Suspected Specimens Using Mouse Monoclonal Antibodies Biologicals. 35. 297- 302